موش تراريخت چیست ؟!

تراريختگي یا ترانس ژنزیز (transgenesis) به فرآيند تغيير در ژنوم با استفاده از تکنیک های DNA نوترکيب گفته مي­شود که شامل موش تراریخت، موش ناک اوت و موش ناک این می باشد.

• موش تراريخت (transgenic mice) به موشی گفته مي شود که ماده ژنتیکی جدید به ژنوم آن اضافه شده و صفت جديدي را کسب نموده است.
• موش ناک اوت (knockout mice) به موشی گفته مي شود که ماده ژنتیکی از ژنوم آن حذف و یا دچار جهش شده است.
• موش ناک این (knockin mice) به موشی گفته مي شود که ماده ژنتیکی از ژنوم آن حذف و ماده ژنتیکی دیگر جایگزین آن شده است.

مجموعه آزمایشگاه های سنجش و پایش کیاژن فارما با داشتن متخصصین خبره امکان تولید انواع مدل های حیوانی به طرق زیر را دارد:

• توليد موش ترانس ژن از طريق ريزتزريقی به پيش هسته (pronucleus injection)
• توليد موش ژن سرکوب شده (knockout mice) از طریق تزریق سلول های بنیادی جنینی به بلاستوسیت یا از طریق تکنیک CRISPR/Cas9
• تولید موش کایمرا (chimeras) از طریق وارد کردن سلول های بنیادی به جنین های مرحله مرولا (morula aggregation)
• طراحي و ساخت سازه­ های ژنی
• کشت و دستکاری ژنتيکی سلول ­های بنيادی جنينی

#موش تراریخته#موش ترانسژنیک#موش ترنسژن#تراریختگی#موش ترانس ژنیک#موش ترنس ژنیک#ترانس ژنزیز#ترانس ژنسیس#transgenic mice#transgenesis#l,a jvhvdoji#l,a jvhvdoj#l,a jvhksCkd;#pd,hk jvhvdoji#pd,hk jvhvdoji#حیوان تراریخته#موش وازکتوم#وازکتوم کردن موش

كاربرد موش ترانسژن چیست ؟!

• ايجاد مدل بيماری های ژنتيكی انسان در موش آزمایشگاهی
• بررسی عملكرد ژن­ ها از طريق حذف یا اضافه كردن ژن­
• مطالعات ژنتيک تكوينی
• بررسی اثر دارو ها جهت يافتن درمان جديد بیماری

#موش تراریخته#موش ترانسژنیک#موش ترنسژن#تراریختگی#موش ترانس ژنیک#موش ترنس ژنیک#ترانس ژنزیز#ترانس ژنسیس#transgenic mice#transgenesis#l,a jvhvdoji#l,a jvhvdoj#l,a jvhksCkd;#pd,hk jvhvdoji#pd,hk jvhvdoji#حیوان تراریخته#موش وازکتوم#وازکتوم کردن موش

مدل های بیماری موجود در بخش رویان مجموعه آزمایشگاه های سنجش و پایش کیاژن فارما شامل موارد زیر می باشد:

• موش سبز (EGFP)
• موش نابارور SPATA19
• موش نابارور ADAM27
• موش ناباروز STRA8
• موش نئومایسین (PGK Neomycin)
• موش هموفیلی (Factor 8)

#موش تراریخته#موش ترانسژنیک#موش ترنسژن#تراریختگی#موش ترانس ژنیک#موش ترنس ژنیک#ترانس ژنزیز#ترانس ژنسیس#transgenic mice#transgenesis#l,a jvhvdoji#l,a jvhvdoj#l,a jvhksCkd;#pd,hk jvhvdoji#pd,hk jvhvdoji#حیوان تراریخته#موش وازکتوم#وازکتوم کردن موش

روش وازکتوم کردن موش چگونه است ؟!

در پروسه تولید موش تراریخت (transgenic mice)، از موش نر وازکتوم (vasectomized mice) جهت آماده سازی موش گیرنده جنین (foster/recipient) استفاده می شود.

• حیوان را بیهوش کنید و در وضعیت پشتی قرار دهید.
• پوست، عضلات و صفاق ناحیه خط میانی در مکان بالاتر از بخش آزاد آلت تناسلی را برش دهید.
• یکی از بیضه ها را خارج کنید.
• وازو دفران (vas deferens) را شناسایی و آن را ببرید یا بسوزانید.
• بیضه را به داخل حفره شکمی برگردانید.
• مراحل را بر روی بیضه دیگر تکرار کنید.
• صفاق، عضلات و پوست را به صورت مجزا بخیه کنید.

#موش تراریخته#موش ترانسژنیک#موش ترنسژن#تراریختگی#موش ترانس ژنیک#موش ترنس ژنیک#ترانس ژنزیز#ترانس ژنسیس#transgenic mice#transgenesis#l,a jvhvdoji#l,a jvhvdoj#l,a jvhksCkd;#pd,hk jvhvdoji#pd,hk jvhvdoji#حیوان تراریخته#موش وازکتوم#وازکتوم کردن موش

استاندارد های زیست سازگاری

ISO 10993-1: Biological Evaluation of Medical Devices – Evaluation and testing within a risk management process.

ISO 10993-2: Biological Evaluation of Medical Devices – Animal welfare requirements.

ISO 10993-3: Biological Evaluation of Medical Devices – Tests for genotoxicity, carcinogenicity and reproductive toxicity.

ISO 10993-4: Biological Evaluation of Medical Devices – Selection of tests for interactions with blood.

ISO 10993-5: Biological Evaluation of Medical Devices – Tests for in vitro cytotoxicity.

ISO 10993-6: Biological Evaluation of Medical Devices – Tests for local effects after implantation.

ISO 10993-7: Biological Evaluation of Medical Devices – Ethylene oxide sterilization residuals.

ISO 10993-8: Biological Evaluation of Medical Devices – Selection and qualification of reference materials for biological tests.

ISO 10993-9: Biological Evaluation of Medical Devices – Framework for identification and quantification of potential degradation products.

ISO 10993-10: Biological Evaluation of Medical Devices – Tests for skin sensitization.

ISO 10993-11: Biological Evaluation of Medical Devices – Tests for systemic toxicity.

ISO 10993-12: Biological Evaluation of Medical Devices – Sample preparation and reference materials.

ISO 10993-13: Biological Evaluation of Medical Devices – Identification and quantification of degradation products from polymeric medical devices.

ISO 10993-14: Biological Evaluation of Medical Devices – Identification and quantification of degradation products from ceramics.

ISO 10993-15: Biological Evaluation of Medical Devices – Identification and quantification of degradation products from metals and alloys.

ISO 10993-16: Biological Evaluation of Medical Devices – Toxicokinetic study design for degradation products and leachables.

ISO 10993-17: Biological Evaluation of Medical Devices – Toxicological risk assessment of medical device constituents.

ISO 10993-18: Biological Evaluation of Medical Devices – Chemical characterization of medical device materials within a risk management process.

ISO 10993-19: Biological Evaluation of Medical Devices – Physico-chemical, morphological and topographical characterization of materials.

ISO 10993-20: Biological Evaluation of Medical Devices – Principles and methods for immunotoxicology testing of medical devices.

ISO 10993-22: Biological Evaluation of Medical Devices – Guidance on nanomaterials.

ISO 10993-23: Biological Evaluation of Medical Devices – Tests for irritation.

OECD Test No. 401: Guidelines for the Testing of Chemicals – Acute Oral Toxicity.

OECD Test No. 402: Guidelines for the Testing of Chemicals – Acute Dermal Toxicity.

OECD Test No. 403: Guidelines for the Testing of Chemicals – Acute Inhalation Toxicity.

OECD Test No. 404: Guidelines for the Testing of Chemicals – Acute Dermal Irritation/Corrosion.

OECD Test No. 405: Guidelines for the Testing of Chemicals – Acute Eye Irritation/Corrosion.

OECD Test No. 406: Guidelines for the Testing of Chemicals – Skin Sensitisation.

OECD Test No. 407: Guidelines for the Testing of Chemicals – Repeated Dose 28-day Oral Toxicity Study in Rodents.

OECD Test No. 408: Guidelines for the Testing of Chemicals – Repeated Dose 90-Day Oral Toxicity Study in Rodents.

OECD Test No. 409: Guidelines for the Testing of Chemicals – Repeated Dose 90-Day Oral Toxicity Study in Non-Rodents.

OECD Test No. 410: Guidelines for the Testing of Chemicals – Repeated Dose Dermal Toxicity: 21/28-day Study.

OECD Test No. 411: Guidelines for the Testing of Chemicals – Subchronic Dermal Toxicity: 90-day Study.

OECD Test No. 412: Guidelines for the Testing of Chemicals – Subacute Inhalation Toxicity: 28-Day Study.

OECD Test No. 413: Guidelines for the Testing of Chemicals – Subchronic Inhalation Toxicity: 90-day Study.

OECD Test No. 414: Guidelines for the Testing of Chemicals – Prenatal Developmental Toxicity Study.

OECD Test No. 415: Guidelines for the Testing of Chemicals – One-Generation Reproduction Toxicity Study.

OECD Test No. 416: Guidelines for the Testing of Chemicals – Two-Generation Reproduction Toxicity.

OECD Test No. 417: Guidelines for the Testing of Chemicals – Toxicokinetics.

OECD Test No. 418: Guidelines for the Testing of Chemicals – Delayed Neurotoxicity of Organophosphorus Substances Following Acute Exposure.

OECD Test No. 419: Guidelines for the Testing of Chemicals – Delayed Neurotoxicity of Organophosphorus Substances: 28-day Repeated Dose Study.

OECD Test No. 420: Guidelines for the Testing of Chemicals – Acute Oral Toxicity – Fixed Dose Procedure.

OECD Test No. 421: Guidelines for the Testing of Chemicals – Reproduction/Developmental Toxicity Screening Test.

OECD Test No. 422: Guidelines for the Testing of Chemicals – Combined Repeated Dose Toxicity Study with the Reproduction/Developmental Toxicity Screening Test.

OECD Test No. 423: Guidelines for the Testing of Chemicals – Acute Oral toxicity – Acute Toxic Class Method.

OECD Test No. 424: Guidelines for the Testing of Chemicals – Neurotoxicity Study in Rodents.

OECD Test No. 425: Guidelines for the Testing of Chemicals – Acute Oral Toxicity: Up-and-Down Procedure.

OECD Test No. 426: Guidelines for the Testing of Chemicals – Developmental Neurotoxicity Study.

OECD Test No. 427: Guidelines for the Testing of Chemicals – Skin Absorption: In Vivo Method.

OECD Test No. 428: Guidelines for the Testing of Chemicals – Skin Absorption: In Vitro Method.

OECD Test No. 429: Guidelines for the Testing of Chemicals – Skin Sensitisation.

OECD Test No. 430: Guidelines for the Testing of Chemicals – In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test Method (TER).

OECD Test No. 431: Guidelines for the Testing of Chemicals – In vitro skin corrosion: reconstructed human epidermis (RHE) test method.

OECD Test No. 432: Guidelines for the Testing of Chemicals – In Vitro 3T3 NRU Phototoxicity Test.

OECD Test No. 433: Guidelines for the Testing of Chemicals – Acute Inhalation Toxicity: Fixed Concentration Procedure.

OECD Test No. 434: Guidelines for the Testing of Chemicals – Acute Dermal Toxicity – Fixed Dose Procedure.

OECD Test No. 435: Guidelines for the Testing of Chemicals – In Vitro Membrane Barrier Test Method for Skin Corrosion.

OECD Test No. 436: Guidelines for the Testing of Chemicals – Acute Inhalation Toxicity – Acute Toxic Class Method.

OECD Test No. 437: Guidelines for the Testing of Chemicals – Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage.

OECD Test No. 438: Isolated Chicken Eye Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage.

OECD Test No. 439: Guidelines for the Testing of Chemicals – In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method.

OECD Test No. 440: Guidelines for the Testing of Chemicals – Uterotrophic Bioassay in Rodents.

OECD Test No. 441: Guidelines for the Testing of Chemicals – Hershberger Bioassay in Rats.

OECD Test No. 442A: Guidelines for the Testing of Chemicals – Skin Sensitization – Local Lymph Node Assay: DA.

OECD Test No. 442B: Guidelines for the Testing of Chemicals – Skin Sensitization – Local Lymph Node Assay: BrdU-ELISA or –FCM.

OECD Test No. 442C: Guidelines for the Testing of Chemicals – In Chemico Skin Sensitisation – Assays addressing the Adverse Outcome Pathway key event on covalent binding to proteins.

OECD Test No. 442D: Guidelines for the Testing of Chemicals – In Vitro Skin Sensitisation – ARE-Nrf2 Luciferase Test Method.

OECD Test No. 442E: Guidelines for the Testing of Chemicals – 442E: In Vitro Skin Sensitisation – In Vitro Skin Sensitisation assays addressing the Key Event on activation of dendritic cells on the Adverse Outcome Pathway for Skin Sensitisation.

OECD Test No. 443: Guidelines for the Testing of Chemicals – Extended One-Generation Reproductive Toxicity Study.

OECD Test No. 451: Guidelines for the Testing of Chemicals – Carcinogenicity Studies.

OECD Test No. 452: Guidelines for the Testing of Chemicals – Chronic Toxicity Studies.

OECD Test No. 453: Guidelines for the Testing of Chemicals – Combined Chronic Toxicity/Carcinogenicity Studies.

OECD Test No. 455: Guidelines for the Testing of Chemicals – Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists.

OECD Test No. 456: Guidelines for the Testing of Chemicals – H295R Steroidogenesis Assay.

OECD Test No. 457: Guidelines for the Testing of Chemicals – BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists.

OECD Test No. 458: Guidelines for the Testing of Chemicals – Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals.

OECD Test No. 460: Guidelines for the Testing of Chemicals – Fluorescein Leakage Test Method for Identifying Ocular Corrosives and Severe Irritants.

OECD Test No. 471: Guidelines for the Testing of Chemicals – Bacterial Reverse Mutation Test.

OECD Test No. 473: Guidelines for the Testing of Chemicals – In Vitro Mammalian Chromosomal Aberration Test.

OECD Test No. 474: Guidelines for the Testing of Chemicals – Mammalian Erythrocyte Micronucleus Test.

OECD Test No. 475: Guidelines for the Testing of Chemicals – Mammalian Bone Marrow Chromosomal Aberration Test.

OECD Test No. 476: Guidelines for the Testing of Chemicals – In Vitro Mammalian Cell Gene Mutation Tests using the Hprt and xprt genes.

OECD Test No. 477: Guidelines for the Testing of Chemicals – Genetic Toxicology: Sex-Linked Recessive Lethal Test in Drosophila melanogaster.

OECD Test No. 478: Guidelines for the Testing of Chemicals – Rodent Dominant Lethal Test.

OECD Test No. 479: Guidelines for the Testing of Chemicals – Genetic Toxicology: In vitro Sister Chromatid Exchange Assay in Mammalian Cells.

OECD Test No. 480: Guidelines for the Testing of Chemicals – Genetic Toxicology: Saccharomyces cerevisiae, Gene Mutation Assay.

OECD Test No. 481: Guidelines for the Testing of Chemicals – Genetic Toxicology: Saacharomyces cerevisiae, Miotic Recombination Assay.

OECD Test No. 482: Guidelines for the Testing of Chemicals – Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells in vitro.

OECD Test No. 483: Guidelines for the Testing of Chemicals – Mammalian Spermatogonial Chromosomal Aberration Test.

OECD Test No. 484: Guidelines for the Testing of Chemicals – Genetic Toxicology: Mouse Spot Test.

OECD Test No. 485: Guidelines for the Testing of Chemicals – Genetic toxicology, Mouse Heritable Translocation Assay.

OECD Test No. 486: Guidelines for the Testing of Chemicals – Unscheduled DNA Synthesis (UDS) Test with Mammalian Liver Cells in vivo.

OECD Test No. 487: Guidelines for the Testing of Chemicals – In Vitro Mammalian Cell Micronucleus Test.

OECD Test No. 488: Guidelines for the Testing of Chemicals – Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays.

OECD Test No. 489: Guidelines for the Testing of Chemicals – In Vivo Mammalian Alkaline Comet Assay.

OECD Test No. 490: Guidelines for the Testing of Chemicals – In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene.

OECD Test No. 491: Guidelines for the Testing of Chemicals – Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage.

OECD Test No. 492: Guidelines for the Testing of Chemicals – Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage.

OECD Test No. 493: Guidelines for the Testing of Chemicals – Performance-Based Test Guideline for Human Recombinant Estrogen Receptor (hrER) In Vitro Assays to Detect Chemicals with ER Binding Affinity.

OECD Test No. 494: Guidelines for the Testing of Chemicals – Vitrigel-Eye Irritancy Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage.

OECD Test No. 495: Guidelines for the Testing of Chemicals – Ros (Reactive Oxygen Species) Assay for Photoreactivity.

OECD Test No. 496: Guidelines for the Testing of Chemicals – In vitro Macromolecular Test Method for Identifying Chemicals Inducing Serious Eye Damage and Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage.

OECD Test No. 497: Guidelines for the Testing of Chemicals – Defined Approaches on Skin Sensitisation.

OECD Test No. 498: Guidelines for the Testing of Chemicals – In vitro Phototoxicity – Reconstructed Human Epidermis Phototoxicity test method.

OECD Test No. 19: Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpoints for Experimental Animals Used in Safety Evaluation. Environmental Health and Safety Monograph Series on Testing and Assessment.

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